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在大鼠附睾中分析microRNA并应用RNA干扰进行基因敲除

小RNA的鉴定、检测和使用在过去的几年中得到了迅猛的发展。我们在小鼠的睾丸中研究了这两个内在相关的领域。首先，通过基因芯片和小RNA文库筛选系统，一组组织特异性和时间短期表达变化的调节性小RNA被鉴定出来。其次，小的双链小干扰RNA，是一种下调基因表达的有力工具。它已经成功地用于活体内附睾基因的表达。

Identification, detection, and use of small RNA molecules have been paid increasing attention in the past decades. Here we show studies of two inter-related lines of this research in a sperm maturation-related organ, the epididymis. First, by using microarray and small RNA library screening systems, a series of spatially- and temporally-regulated known and novel small regulatory RNA molecules, referred to as microRNAs has been identified and characterized. Second, small double-stranded RNAs, called small interfering RNAs, emerged as a powerful tool to knock-down gene expression by a pathway known as RNA interference. It has been successfully used to silence epididymal gene expression in vivo to elucidate the function of several sperm motility- and capacitation-related proteins in the rat epididymis.

J Androl. 2011 Jul 15.