• 慢病毒载体构建
• 细胞亚定位慢病毒载体
• 慢病毒纯化试剂
• 慢病毒载体
• 慢病毒包装服务
• 慢病毒包装体系
• 信号通路研究慢病毒载体

慢病毒表达体系

    慢病毒属于逆转录病毒的一种。慢病毒感染细胞后将外源基因整合到细胞基因组，从而实现基因的持续表达。慢病毒表达体系由于整合率高、感染谱广、表达稳定，尤其适合用于稳定细胞系构建。我们的慢病毒产品包括慢病毒载体、慢病毒包装体系和纯化试剂。

特点

1、将外源基因整合到基因组实现稳定表达。

2、整合率高，表达稳定。

3、容量可达5kB以上（包括启动子等）。

客户使用本产品发表的部分文献

• Sun, Y. X., Xu, A. H., Yang, Y., Zhang, J. X., & Yu, A. W. (2015). Activation of cannabinoid receptor 2 enhances osteogenic differentiation of bone marrow derived mesenchymal stem cells. BioMed research international, 2015.
• Zhang, Y., Wu, S., Song, S., Lv, J., Feng, C., & Lin, X. (2015). Preparation and characterization of a stable BHK-21 cell line constitutively expressing the Schmallenberg virus nucleocapsid protein. Molecular and cellular probes, 29(4), 244-253.
• Wang, J., Kang, W. M., Yu, J. C., Liu, Y. Q., Meng, Q. B., & Cao, Z. J. (2013). Cadherin-17 induces tumorigenesis and lymphatic metastasis in gastric cancer through activation of NFκB signaling pathway. Cancer biology & therapy, 14(3), 262-270.
• Yin, X., Yi, H., Wang, L., Wu, W., Wu, X., & Yu, L. (2016). RSPOs facilitated HSC activation and promoted hepatic fibrogenesis. Oncotarget, 7(39), 63767.
• Zhang, Y., Wu, S., Song, S., Lv, J., Feng, C., & Lin, X. (2017). Generation and characterization of a potentially applicable Vero cell line constitutively expressing the Schmallenberg virus nucleocapsid protein. Cytotechnology, 69(1), 145-156.
• Zhao, H., Tang, H., Huang, Q., Qiu, B., Liu, X., Fan, D., ... & Yang, L. (2016). MiR-101 targets USP22 to inhibit the tumorigenesis of papillary thyroid carcinoma. American journal of cancer research, 6(11), 2575.
• Qin, L., Deng, H. Y., Chen, S. J., Wei, W., & Zhang, Y. T. (2017). miR-139 acts as a tumor suppressor in T-cell acute lymphoblastic leukemia by targeting CX chemokine receptor 4. American journal of translational research, 9(9), 4059.
• Cheng, Z., Li, Z., Ma, K., Li, X., Tian, N., Duan, J., ... & Wang, Y. (2017). Long Non-coding RNA XIST Promotes Glioma Tumorigenicity and Angiogenesis by Acting as a Molecular Sponge of miR-429. Journal of Cancer, 8(19), 4106.
• Xiao, X., Tang, W., Yuan, Q., Peng, L., & Yu, P. (2015). Epigenetic repression of Krüppel-like factor 4 through Dnmt1 contributes to EMT in renal fibrosis. International journal of molecular medicine, 35(6), 1596-1602.
• Wang, Z. L., Deng, Q., Chong, T., & Wang, Z. M. (2018). Autophagy suppresses the proliferation of renal carcinoma cell. European review for medical and pharmacological sciences, 22, 343-350.
• Zhang, D. D., Wang, W. T., Xiong, J., Xie, X. M., Cui, S. S., Zhao, Z. G., ... & Li, Y. J. (2017). Long noncoding RNA LINC00305 promotes inflammation by activating the AHRR-NF-κB pathway in human monocytes. Scientific reports, 7, 46204.
• Wang, P., Shi, B., GAO, Z., Sun, T. F., YANG, W., Han, S. F., ... & WANG, D. (2016). EFFECT OF COLLA CORNUS CERVI COMBINED WITH LV-MEDIATED BMP7 TRANSFECTED BMSCs ON ANFH IN RATS. ACTA POLONIAE PHARMACEUTICA, 73(6), 1521-1530.
• Ma, X., Gu, L., Li, H., Gao, Y., Li, X., Shen, D., ... & Fan, Y. (2015). Hypoxia-induced overexpression of stanniocalcin-1 is associated with the metastasis of early stage clear cell renal cell carcinoma. Journal of translational medicine, 13(1), 56.
• Du, Z., Dong, C., & Ren, J. (2017). A study of the dynamics of PTEN proteins in living cells using in vivo fluorescence correlation spectroscopy. Methods and applications in fluorescence, 5(2), 024008.
• Pan, Z., Ding, Q., Guo, Q., Guo, Y., Wu, L., Wu, L., ... & Zhou, F. (2018). MORC2, a novel oncogene, is upregulated in liver cancer and contributes to proliferation, metastasis and chemoresistance. International journal of oncology.
• Li, C., Du, S., Tian, M., Wang, Y., Bai, J., Tan, P., ... & Li, Y. (2018). The host restriction Factor interferon-inducible Transmembrane Protein 3 inhibits Vaccinia Virus infection. Frontiers in Immunology, 9, 228.
• Gao, Y., Li, H., Ma, X., Fan, Y., Ni, D., Zhang, Y., ... & Gu, L. Y. (2016). KLF6 suppresses metastasis of clear cell renal cell carcinoma via transcriptional repression of E2F1. Cancer research, canres-0348.
• Li, X., Liu, N., Wang, Y., Liu, J., Shi, H., Qu, Z., ... & Gu, B. (2017). Brain and muscle aryl hydrocarbon receptor nuclear translocator-like protein-1 cooperates with glycogen synthase kinase-3β to regulate osteogenesis of bone-marrow mesenchymal stem cells in type 2 diabetes. Molecular and cellular endocrinology, 440, 93-105.
• Yin, X., Yi, H., Wang, L., Wu, W., Wu, X., & Yu, L. (2017). R-spondin 2 promotes proliferation and migration via the Wnt/β-catenin pathway in human hepatocellular carcinoma. Oncology letters, 14(2), 1757-1765.

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RNA干扰 基因合成 基因克隆 慢病毒 腺病毒 腺相关病毒 细胞系服务 蛋白表达 Polyfect-v转染试剂 TALEN技术