慢病毒表达体系

    慢病毒属于逆转录病毒的一种。慢病毒感染细胞后将外源基因整合到细胞基因组，从而实现基因的持续表达。慢病毒表达体系由于整合率高、感染谱广、表达稳定，尤其适合用于稳定细胞系构建。我们的慢病毒产品包括慢病毒载体、慢病毒包装体系和纯化试剂。

特点

1、将外源基因整合到基因组实现稳定表达。

2、整合率高，表达稳定。

3、容量可达5kB以上（包括启动子等）。

客户使用本产品发表的部分文献

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• Zhang, Y., Wu, S., Song, S., Lv, J., Feng, C.,  & Lin, X. (2015). Preparation and characterization of a stable BHK-21 cell  line constitutively expressing the Schmallenberg virus nucleocapsid protein. Molecular and cellular probes, 29(4),  244-253.

• Wang, J., Kang, W. M., Yu, J. C., Liu, Y. Q.,  Meng, Q. B., & Cao, Z. J. (2013). Cadherin-17 induces tumorigenesis and  lymphatic metastasis in gastric cancer through activation of NFκB signaling  pathway. Cancer biology &  therapy, 14(3),  262-270.

• Yin, X., Yi, H., Wang, L., Wu, W., Wu, X.,  & Yu, L. (2016). RSPOs facilitated HSC activation and promoted hepatic  fibrogenesis. Oncotarget, 7(39), 63767.

• Zhang, Y., Wu, S., Song, S., Lv, J., Feng, C.,  & Lin, X. (2017). Generation and characterization of a potentially applicable  Vero cell line constitutively expressing the Schmallenberg virus nucleocapsid  protein. Cytotechnology, 69(1), 145-156.

• Zhao, H., Tang, H., Huang, Q., Qiu, B., Liu,  X., Fan, D., ... & Yang, L. (2016). MiR-101 targets USP22 to inhibit the  tumorigenesis of papillary thyroid carcinoma. American journal of  cancer research, 6(11),  2575.

• Qin, L., Deng, H. Y., Chen, S. J., Wei, W.,  & Zhang, Y. T. (2017). miR-139 acts as a tumor suppressor in T-cell acute  lymphoblastic leukemia by targeting CX chemokine receptor 4. American journal of translational research, 9(9), 4059.

• Cheng, Z., Li, Z., Ma, K., Li, X., Tian, N.,  Duan, J., ... & Wang, Y. (2017). Long Non-coding RNA XIST Promotes Glioma  Tumorigenicity and Angiogenesis by Acting as a Molecular Sponge of miR-429. Journal of Cancer, 8(19),  4106.

• Xiao, X., Tang, W., Yuan, Q., Peng, L., &  Yu, P. (2015). Epigenetic repression of Krüppel-like factor 4 through Dnmt1  contributes to EMT in renal fibrosis. International  journal of molecular medicine, 35(6),  1596-1602.

• Wang, Z. L., Deng, Q., Chong, T., & Wang,  Z. M. (2018). Autophagy suppresses the proliferation of renal carcinoma cell. European review for medical and pharmacological sciences, 22, 343-350.

• Zhang, D. D., Wang, W. T., Xiong, J., Xie, X.  M., Cui, S. S., Zhao, Z. G., ... & Li, Y. J. (2017). Long noncoding RNA  LINC00305 promotes inflammation by activating the AHRR-NF-κB pathway in human  monocytes. Scientific reports, 7, 46204.

• Wang, P., Shi, B., GAO, Z., Sun, T. F., YANG,  W., Han, S. F., ... & WANG, D. (2016). EFFECT OF COLLA CORNUS CERVI  COMBINED WITH LV-MEDIATED BMP7 TRANSFECTED BMSCs ON ANFH IN RATS. ACTA POLONIAE PHARMACEUTICA, 73(6),  1521-1530.

• Ma, X., Gu, L., Li, H., Gao, Y., Li, X., Shen,  D., ... & Fan, Y. (2015). Hypoxia-induced overexpression of stanniocalcin-1  is associated with the metastasis of early stage clear cell renal cell  carcinoma. Journal of translational  medicine, 13(1), 56.

• Du, Z., Dong, C., & Ren, J. (2017). A study  of the dynamics of PTEN proteins in living cells using in vivo fluorescence  correlation spectroscopy. Methods and  applications in fluorescence, 5(2),  024008.

• Pan, Z., Ding, Q., Guo, Q., Guo, Y., Wu, L.,  Wu, L., ... & Zhou, F. (2018). MORC2, a novel oncogene, is upregulated in  liver cancer and contributes to proliferation, metastasis and chemoresistance. International journal of oncology.

• Li, C., Du, S., Tian, M., Wang, Y., Bai, J.,  Tan, P., ... & Li, Y. (2018). The host restriction Factor  interferon-inducible Transmembrane Protein 3 inhibits Vaccinia Virus infection. Frontiers in Immunology, 9, 228.

• Gao, Y., Li, H., Ma, X., Fan, Y., Ni, D.,  Zhang, Y., ... & Gu, L. Y. (2016). KLF6 suppresses metastasis of clear cell  renal cell carcinoma via transcriptional repression of E2F1. Cancer research, canres-0348.

• Li, X., Liu, N., Wang, Y., Liu, J., Shi, H.,  Qu, Z., ... & Gu, B. (2017). Brain and muscle aryl hydrocarbon receptor  nuclear translocator-like protein-1 cooperates with glycogen synthase kinase-3β  to regulate osteogenesis of bone-marrow mesenchymal stem cells in type 2  diabetes. Molecular and  cellular endocrinology, 440, 93-105.

• Yin, X., Yi, H., Wang, L., Wu, W., Wu, X.,  & Yu, L. (2017). R-spondin 2 promotes proliferation and migration via the  Wnt/β-catenin pathway in human hepatocellular carcinoma. Oncology letters, 14(2),  1757-1765.

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