΢ɨһɨ

˵

ŻϢ


CRISPR/Cas9

ϸϵ
circRNA
Ʒ

ѯ


*ʼ

*

*λ

*ȤķƷ

ϸ˵

壿

ҳ > > >

Ӣïʢҵϸ༭شͻƣͻ1.6Ԫ/

ǩ ʱ䣺2019-10-17

Ӣïʢҵϸ༭شͻƣͻ1.6 Ԫ/

Ӣïʢҵϸóȡشͻƣϸ༭ɱΪͻչϸóŻݣ

 

  1.   ۸񼫵——1.6Ԫ/
  2.   ɹʸ——ɹշѣ
  3.   ڶ——8-10
  4.   ——ṩĵ¡ϸ
  5.   Ӧù㷺——鶯ϸó

 

ԭ
DzCas9޸ںϼǴϸó޸Чʡͨ¼ǽϸó2ϣЧ10ϣͬʱϸóɱ½

ͼ1 Cas9-HRںϵ׽鵼༭ԭ

 

 

Ӧã
EGFP293T-mito-mcherryϸ
293T-mito-mcherryϸǰȶ嶨λmcherryӫ⵰ϸ꣬ϸȶmito-mcherryǸmito-mcherryУmcherryCas9еEGFP2ABsd޸ģ塣ͨssDNAģ塢Cas9ںϵסsgRNAת293T-mito-mcherryϸ꣬blasticidinɸѡô¡ӡȷʴ90%
ʵƣ


ͼ2 EGFPmcherryʵ

 



ͼ3 DNA PCRMDL2000 marker1δ༭ϸ2~7༭󵥿¡ϸ5ſ¡Ӻ⣬ΪԿ¡

 

ͼ4  3ſ¡ӫʾmcherryEGFPλͬ

 

 

ѯߣ
QQ1291769782
΢/ֻ13681365274
Tel023-67630383
ӢïʢҵƼ޹˾


  • һƪsgRNAת¼ж򵥣
  • һƪû

  • Ȩ ӢïʢҵƼ޹˾ վICP19010777
    ַн֧·4ž۷C7¥ 绰:023-67630383
    Ʒorder@inovogen.com Ʒѯmarket@inovogen.com

    Cas9/óȶϸϵ/תϸ תϸϵֻƷ